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RPGVX Reader

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RPGVX Reader

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The aim of this study was to explore whether autophagy activation mediates the effects of thyroid hormone on skeletal muscle growth. Autophagy was assessed in mouse skeletal muscle treated in vivo with T3, a natural thyroid hormone. The formation of autophagosomes and the autophagy flux were studied using electron microscopy and immunoblotting. The mTORC1 signaling pathway was evaluated by evaluating p-mTOR, mTOR, p-4EBP1 and 4EBP1 protein expression levels. The effect of T3 on mTORC1 signaling was also evaluated in vitro on L6 myotubes incubated with T3 and on mouse embryonic myoblasts overexpressing mRFP-GFP-LC3. We found that T3 treatment promoted autophagy by activating the mTORC1 signaling pathway. In addition, T3 also increased the LC3-II/LC3-I ratio and LC3 puncta number. T3-induced autophagy is associated with reduced p62/SQSTM1 protein levels and increased p-Akt and p-mTOR levels. Finally, we observed that T3 treatment induces hypertrophy in vitro and in vivo. In conclusion, our data indicate that T3 induces skeletal muscle hypertrophy and growth by mediating autophagy. Moreover, T3 promotes both the formation and flux of autophagosomes.Non-adaptive response of genetic variation to selection for disease resistance and growth in the domestic chicken.
Despite advances in the understanding of the genetic bases for the immune system, little is known about the genetic basis of resistance to infectious diseases. This study analysed the response of quantitative and qualitative genetic variation to 12 years of artificial selection for broiler performance, growth rate and survival to infectious bronchitis virus (IBV) for a population of chickens. Genetic effects were determined using three different models: univariate animal models, bivariate animal models and bivariate regression. In all models, heritabilities for IBV resistance were low (

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