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Download Windows XP Pro Service Pack 3 SP3 – Windows .1. Field of the Invention
The present invention relates to the field of the detection of particular sequences, including, but not limited to, DNA sequences in the presence of single nucleotide polymorphism.
2. Related Art
The identification and/or isolation of nucleic acid (including, for example, DNA and RNA) sequences that encode gene products of interest is fundamental to modem genetic engineering. Examples of gene products of interest include, but are not limited to, antigens, receptors, and enzymes. Such gene products may be identified using sequence based methods, as well as other methods known in the art.
In many cases, it is desirable to detect or identify nucleic acid sequences that differ from a reference sequence. In some cases, these differences result in the change of a single nucleotide base in a DNA or RNA sequence. Such single nucleotide polymorphisms (SNPs) are useful in the genetic analysis of animals and humans, as well as in the genetic and physical mapping of organisms. For example, DNA polymorphisms may be used to detect and characterize mutations, for determining the inheritance of a particular genotype, for matching samples and for genetic fingerprinting.
One method for detecting sequence differences among nucleic acids is known as single strand conformational polymorphism (SSCP). See, e.g., Huang et al. (1991) Genomics 14:667-674; Tazi-Ahnini et al. (1991) EMBO J. 10:4899-4904; Wu et al. (1991) Genomics 14:704-709
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