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The effects of salinity on the cell growth, physiological and morphological characteristics of alfalfa (Medicago sativa L.) cells were examined. The experimental growth medium was prepared by diluting Hoagland’s solution, containing various concentrations of NaCl (0, 50, 100 and 200 mM). The experiments were performed in a plant growth chamber, maintained at 21 +/- 2 degrees C, 50 +/- 10% relative humidity, and under a 14/10-h light/dark regime. The alfalfa seedlings (c.a. 3 weeks old) were transferred to fresh growth medium each day. After 4 days of culture, alfalfa seedlings were treated with 50 mM NaCl (salt stress) for 24 h and 1 or 2 days. The highest salt stress tolerance was observed in alfalfa seedlings cultured in the medium containing 200 mM NaCl for 2 days. The growth of alfalfa seedlings was greatly retarded by salt stress. The amount of soluble protein increased by salt stress, but was not changed by the salt stress over a two-day period. It was suggested that salinity enhanced the tolerance of alfalfa seedlings to salt stress.Q:
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In java applet, we can do
Applet app = getApplet();
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app.getEventDispatcher(),
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e.getModifiers(),
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e.getY(),
e.getClickCount(),
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