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Migration and expansion of myocardial interstitial cells (MICs) is involved in the origin of fibrosis and cardiac remodelling. The aim of this study was to evaluate the expression of ICAM-1, ICAM-2, ICAM-3, and CD44 on the myocardial cell surface of the infarcted and peri-infarct areas. The expression of ICAM-1 and CD44 was evaluated in the infarcted area (I) of adult rabbits and in areas of normal myocardium surrounding the area at risk (A) and the infarcted area (CA), using immunohistochemistry (IHC). Fibrosis was also evaluated by measuring hydroxyproline in the infarcted area and by immunoblotting analysis of fibronectin expression. Myocardial cell adhesion molecule expression was evaluated on the membrane and cytoplasm of cardiomyocytes, and on the membrane and pericellular matrix of collagen-containing cells, using immunogold and immunogold-silver methods. ICAM-1, ICAM-2, ICAM-3, and CD44 were expressed in the membrane and cytoplasm of cardiomyocytes. The IHC results showed the expression of ICAM-1, ICAM-2, and ICAM-3 in cardiomyocytes of infarcted area, A and CA, without any
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Ten patients with primary acromegaly were treated with bromocriptine in order to evaluate the efficacy of this drug on growth hormone-releasing hormone (GRH) release. Blood samples were taken at regular intervals for the measurement of plasma growth hormone, insulin, and GRH. A significant decrease of GH levels from 54.3 to 11.0 mU/l (p less than 0.001) was seen at 1 month after starting bromocriptine therapy. At the same time, the plasma GRH concentration increased from 33.5 to 97 pg/ml (p less than 0.001) and remained almost unchanged during the following months. Blood glucose levels decreased from 4.9 to 4.1 mmol/liter (p less than 0.01). The rate of growth in the group of 8 patients was reduced from 16.0 to 7.2 cm/year. Bromocriptine has proved to be an efficacious therapy in most of our patients. However, because of the marked hypoglycemia, the therapeutic effect of bromocriptine will probably be maximal in patients with early, rapidly progressing and severe disease.Q:
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