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Disclaimer : The information contained on this website is not financial advice nor legal advice. The content on this website does not reflect the views of Company and the information on the website should not be interpreted as financial or legal advice.Uptake of riboflavin by rat hepatocytes, with an emphasis on membranes bound to organelles: effect of metabolic activation.
Rat hepatocytes were incubated with riboflavin-5-phosphate, and the incorporation of the vitamin was investigated. The primary fate of the vitamin was therefore its incorporation into the plasma membrane. More than half of the total uptake was inhibited by the presence of superoxide dismutase, indicating a possible participation of a membrane-associated redox system in the riboflavin uptake. The other major uptake route was the one of the endocytosis type, which represented more than 70% of the riboflavin accumulated by the cells. The endocytosed riboflavin was distributed in the lysosomal, mitochondrial, and rough endoplasmic reticulum membranes. This pattern of endocytosis was similar to that already observed for lipid-derived hormones, and we suggest that riboflavin may also be used as a marker for these membranes. The metabolism of the intracellular riboflavin occurred mainly in the mitochondria, but part of the metabolized vitamin was probably transferred to the endoplasmic reticulum. This could have an important significance since the endoplasmic reticulum is considered to participate in the formation of other membrane structures, and in the secretion of membrane-associated proteins.Telephone operators worldwide are becoming increasingly challenged to provide new services to existing customers while at the same time developing new services for the telephone industry as a whole.
One area in which operators are developing new services is in the area of adding telephone-like services to non-telephone communications networks. Such non-telephone communications networks include general purpose computer networks which are typically connected
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