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November 29, 2022

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an abstract should present the new scientific findings, not a summary of the background of the field. for most journals, the exception is the journal of contemporary applied microbiology for which the abstract also includes a detailed narrative. the abstract starts with a ‘we’ statement followed by the generic description of the experiments, then highlighting the overarching result. the abstract ends with a conclusion that highlights the practical utility of the work and a call for further study.

coupled with tissue engineering, silk fibroin scaffolds are a promising material for the application of induced pluripotent stem cells (ips) ¹. in this study, we evaluated the impact of culturing on the mechanical and morphological properties of silk fibroin hydrogels, as well as the efficiency and effectiveness of the ips cell culture on the composite scaffold. the mechanical properties of hydrogel samples were analyzed using compression tests. cell migration on the scaffold was observed using live/dead staining and scanning electron microscopy (sem). the ability of ips cells to grow and differentiate into cardiomyocytes in vitro on the scaffold was evaluated by immunofluorescence staining. the contractile ability of the scaffold was also evaluated using a contraction-relaxation experiment. the results indicated that the mechanical properties of the samples changed with increase of silk fibroin concentration and culturing. the electrical field and culture medium were not found to significantly affect cell viability and migration.

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