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A serial number means a unique copy, a number. A serial number is numerated within the context of a system, such as a. Certification Issue Number Number of Item(s) or Serial Number I.
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AM LIVE SERIAL No.: Â 421669676773
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05-10-2015 06:50PM
2018-04-14
SERIAL NUMBER – AM LIVE 5449199688446
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i am alive serial pcHigh-throughput method for purifying protein-RNA complexes.
Protein-RNA complexes are widespread and important throughout the cell. Subtractive methods, like ‘Transcriptional Shutdown’ and ‘Phage Search and Bind (PSB)’ for purifying protein-RNA complexes, have led to the identification of many protein-RNA complexes from organisms ranging from bacteria to human. In contrast, subtraction methods can be laborious and time consuming. The main objective of the work presented here is to develop a high-throughput method for purifying protein-RNA complexes. This method is used to identify the protein targets of a short RNA with a library of clones containing all possible RNA sequences. Using this method, a total of 46 protein-RNA complexes from Escherichia coli were identified with a protein target accuracy of 95%. A simplified procedure is also described to isolate protein-RNA complexes in mouse and Arabidopsis. The effectiveness and simplicity of this method suggest that it can be used as a high-throughput method for identifying protein
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